Archer’s annual STEM Symposium is an opportunity for Los Angeles high school students who have completed independent or small group projects in STEM to present their findings. Students are invited to present their research in the fields of molecular biology, chemistry, physics, and engineering.

The Archer RISE Award honors exceptional young women for their innovative scientific research or engineering design projects that further existing knowledge or develop solutions to pressing scientific challenges. Finalists for the RISE Award are noted in throughout the online program and will be awarded at lunch on May 21.

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Saturday, May 21 • 9:15am - 10:30am
The Effects of Epigallocatechin-3-Gallate and Ginsenoside Rg3 on Cell Viability and Apoptosis

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Previous research indicates that regulated apoptotic machinery is responsible for suppressing tumor growth and correlates with Chronic Myeloid Leukemia (CML) cell growth. Cells will be tested exposed to the two flavonoids in order to directly see the correspondence between the antioxidants and the cancer line. Cell viability and apoptosis will be measured quantitatively using a flow cytometer, and qualitatively using a microscopic imaging. In addition to testing the flavonoids’ effects on leukemia cells, anti-oxidant properties will be examined using spectrometry to determine if they are auto-oxidative or if they are reacting with the properties of the cancerous cell line. It is thought that the outcome of this portion of the experiment will result in the cancer cells eradicating in a dose dependent matter and not be auto-oxidative. Moreover, research has also suggested that the p53 gene’s activation is the key in apoptotic machinery that suppresses the tumor growth correlated with cell growth. Using this information, this study will use epigallocatechin-3-gallate and ginsenoside Rg3 to activate the p53 gene in mutated gld-1 Caenorhabditis elegans to regulate apoptosis in addition to testing directly on leukemia cells. To detect tumor growth, orange acridine dye and fluorescent microscopy will be used. The expected outcome of this research is finding tumor regression and suppression in C. elegans using epigallocatechin-3-gallate (EGCG) and ginsenoside Rg3 to reinforce the hypothesis that the p53 gene is the source for cancer cell growth.


Seaf Hartley

The Archer School for Girls
The Archer School for Girls; Grade 12

Saturday May 21, 2016 9:15am - 10:30am
Dining Hall