Archer’s annual STEM Symposium is an opportunity for Los Angeles high school students who have completed independent or small group projects in STEM to present their findings. Students are invited to present their research in the fields of molecular biology, chemistry, physics, and engineering.
The Archer RISE Award honors exceptional young women for their innovative scientific research or engineering design projects that further existing knowledge or develop solutions to pressing scientific challenges. Finalists for the RISE Award are noted in throughout the online program and will be awarded at lunch on May 21.
The objective of this research was to test if Muscadine Grape seed extract had an effect on lowering triglyceride levels in C. elegans. By testing Muscadine Grape seed extract's effect on lowering triglyceride content levels, Muscadine Grape seed extract[?]s potential as a treatment for obesity could be determined.For this research both the N2 wild type and tub-1 mutant C. elegans, which model the presence of obesity in the body, were utilized. The tub-1 mutant, which lacked the ability to regulate lipid or fat accumulation and as a result had excess body fat, showed the effects of Muscadine Grape seed extract as a treatment for obesity by lowering the C. elegan’s fat levels. This experiment used a Triglyceride Colorimetric Assay which measured Triglyceride, the main constituent of animal fat, amounts in the body. In the assay, Triglyceride was broken down into free fatty acids and glycerol. Then the plate reader read the sample to determine the amount of glycerol there was in each well, which correlated to how much Triglyceride there was in each well. Each well contained homogenized C. elegans fed either 5%, 10%, or 15% Muscadine Grape seed extract or no Muscadine Grape seed extract at all (Control). This meant that the assay showed the difference in triglyceride content between the different doses of Muscadine Grape seed extract, determining if Muscadine Grape seed extract was treatment for obesity and lowered Triglyceride levels in a dose dependent manner.